Central Cholinergic Synaptic Vesicle Loading Obeys the Set-point Model in Drosophila 2 3
نویسندگان
چکیده
1 Central cholinergic synaptic vesicle loading obeys the set-point model in Drosophila 2 3 Francesca Cash, Samuel W Vernon, Pauline Phelan, Jim Goodchild 3 and Richard A Baines 4 Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom. 5 School of Biosciences, University of Kent, Kent, CT2 7NZ, United Kingdom. 6 Syngenta Crop Protection Research, Bracknell, Berkshire, United Kingdom. 7 8 Pages: 21 9 Figures: 5 10 Tables: 0 11 12 13 14 Running Title: Loading of central cholinergic synaptic vesicles 15 16 17 Address for correspondence: 18 Richard Baines 19 Faculty of Life Sciences, 20 University of Manchester, 21 Manchester, 22 United Kingdom. 23 [email protected] 24 Tel: + 44 161 2755365 25 26 27 28
منابع مشابه
Central cholinergic synaptic vesicle loading obeys the set-point model in Drosophila.
Experimental evidence shows that neurotransmitter release, from presynaptic terminals, can be regulated by altering transmitter load per synaptic vesicle (SV) and/or through change in the probability of vesicle release. The vesicular acetylcholine transporter (VAChT) loads acetylcholine into SVs at cholinergic synapses. We investigated how the VAChT affects SV content and release frequency at c...
متن کاملSynaptic vesicle cycling is not impaired in a glutamatergic and a cholinergic synapse that exhibit deficits in acidification and filling
The purpose of the present work was to investigate synaptic vesicle trafficking when vesicles exhibit alterations in filling and acidification in two different synapses: a cholinergic frog neuromuscular junction and a glutamatergic ribbon-type nerve terminal in the retina. These synapses display remarkable structural and functional differences, and the mechanisms regulating synaptic vesicle cyc...
متن کاملLocalization and Mobility of Synaptic Vesicles in Myosin VI Mutants of Drosophila
BACKGROUND At the Drosophila neuromuscular junction (NMJ), synaptic vesicles are mobile; however, the mechanisms that regulate vesicle traffic at the nerve terminal are not fully understood. Myosin VI has been shown to be important for proper synaptic physiology and morphology at the NMJ, likely by functioning as a vesicle tether. Here we investigate vesicle dynamics in Myosin VI mutants of Dro...
متن کاملIndividual synaptic vesicles from the electroplaque of Torpedo californica, a classic cholinergic synapse, also contain transporters for glutamate and ATP
The type of neurotransmitter secreted by a neuron is a product of the vesicular transporters present on its synaptic vesicle membranes and the available transmitters in the local cytosolic environment where the synaptic vesicles reside. Synaptic vesicles isolated from electroplaques of the marine ray, Torpedo californica, have served as model vesicles for cholinergic neurotransmission. Many lin...
متن کاملFast excitatory synaptic transmission mediated by nicotinic acetylcholine receptors in Drosophila neurons.
Difficulty in recording from single neurons in vivo has precluded functional analyses of transmission at central synapses in Drosophila, where the neurotransmitters and receptors mediating fast synaptic transmission have yet to be identified. Here we demonstrate that spontaneously active synaptic connections form between cultured neurons prepared from wild-type embryos and provide the first dir...
متن کامل